Diagnostic applications of double D-loop formation
US5273881A · kind A · utility
98Cited by
3References
35Claims
0Family size
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Inventors
Key dates
| Filing date | Sep 4, 1991 |
| Grant date | Dec 28, 1993 |
| Priority date | — |
| Expiry date | Sep 4, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention describes the formation of RecA protein catalyzed double-stranded probe:duplex linear target DNA complexes that are stable to deproteinization. The uses of this stable probe:target complex in diagnostic/DNA detection systems in in vitro and in situ DNA hybridization reactions is discussed. The probe:target complexes are also useful for diagnostic application in RecA protein facilitated DNA amplification reactions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.