Method and kit for performing nucleic acid hybridization assays
US5273882A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Nov 20, 1991 |
| Grant date | Dec 28, 1993 |
| Priority date | — |
| Expiry date | Nov 20, 2011 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/532
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and a kit for the isolation and quantitative detection of a selected target nucleic acid sequence from solution employing two probes. A first probe is complementary to one portion of the target and is covalently attached to a first complexing agent (e.g., either an antigen or an antibody). The second probe is complementary to a different portion of the target and is associated with a reporter group. Following hybridization of the target and two probes in solution, a solid support coated with a second complexing agent (i.e., a corresponding antibody or antigen) capable of binding to the first complexing agent on the first probe is employed to immobilize the target-probe hybrid complex. A plurality of types of first probes may be used. Each type is attached to the same sort of complexing agent but each includes a nucleic acid sequence which is complementary to a different portion of the target.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.