Phosphinothricin-resistance gene, and its use

US5273894A · kind A · utility

Assignee

• Hoechst GmbH · DE

Inventors

• Eckhard Strauch · Bielefeld, DE
• Wolfgang Wohlleben · Bielefeld, DE
• Walter Arnold · Bielefeld, DE
• Renate Alijah · Bielefeld, DE
• Alfred Puhler · Bielefeld, DE
• Gerhard Wohner · Flörsheim, DE
• Rudiger Marquardt · Frankfurt am Main, DE
• Susanne Grabley · Königstein, DE
• Dieter Brauer · Flörsheim, DE
• Klaus Bartsch · Steinbach, DE

Key dates

• Filing date: Nov 20, 1991
• Grant date: Dec 28, 1993
• Expiry date: Nov 20, 2011

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8277
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Selection of Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistance selectants. The DNA fragment which carries the phosphinothricin(PTC)-resistance gene is obtained from the total DNA of these selectants by cutting with BamHI, cloning of a fragment 4.0 kb in size, and selection for PTT resistance. This gene is suitable for the production of PTC-resistant plants, and as a resistance marker and for the selective N-acetylation of the L-form of racemic PTC.