Methods for detecting the effect of cell affecting agents on living cells

US5278048A · kind A · utility

Assignee

• Molecular Devices, LLC · US

Inventors

• John Wallace Parce · Palo Alto, US
• Harden M. McConnell · Stanford, US
• Gillian M. K. Humphries · Los Altos, US
• Karen M. Kercso · Menlo Park, US
• John C. Owicki · Palo Alto, US
• Josef E. Kercso · Palo Alto, US

Key dates

• Filing date: May 29, 1991
• Grant date: Jan 11, 1994
• Expiry date: May 29, 2011

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/806
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods are disclosed for detecting the effects of cell affecting agents on living cells. The method steps include providing living cells that are retained in a micro flow chamber. The micro flow chamber is adapted for either continuous or intermittent flow of solutions or suspensions in intimate contact with the cells. The solutions or suspensions, which contain a cell affecting agent, are then flowed in intimate contact with the cells and at least one effect of the cell affecting agent on the cells is measured by an appropriate detecting means, which is operatively associated with the micro flow chamber.