Retroviral packaging cell lines and process of using same

US5278056A · kind A · utility

Assignee

• The Trustees of Columbia University in the City of New York · US

Inventors

• Arthur Bank · Yonkers, US
• Dina G. Markowitz · New York, US
• Stephen P. Goff · Orefield, US

Key dates

• Filing date: Apr 29, 1992
• Grant date: Jan 11, 1994
• Expiry date: Apr 29, 2012

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2740/13052
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a mammalian cell line useful for retroviral packaging comprising two plasmids, the first such plasmid comprising in 5' to 3' order: a DNA sequence comprising a 5' long terminal repeat (LTR); a mutated .psi. packaging sequence; a DNA sequence comprising the encoding portion of the Moloney murine leukemia virus (MULV) env gene; and the second such plasmid comprising in 5' to 3' order: a DNA sequence comprising a 5' long terminal repeat (LTR); a mutated .psi. packaging sequence; a DNA sequence comprising the encoding portion of the Moloney murine leukemia virus (MULV) gag and pol genes; a selectable marker; and an origin of replication. The invention also relates to processes for preparing a producer cell line useful for transferring a gene of interest into recipient mammalian cells in vitro.