Method and reagent composition for the determination of alanine aminotrasferase and HBsAg antigen in the same biological specimen
US5279944A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 15, 1991 |
| Grant date | Jan 18, 1994 |
| Priority date | — |
| Expiry date | May 15, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/82
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The enzyme alanine aminotransferase (ALT) is colorimetrically determined as the hydrogen peroxide obtained in the pyruvate hydrolysis reaction catalysed by the enzyme pyruvate oxidase which develops a colour read at 550 nm making use of a reagent composition buffered at pH comprised within 7.0 and 7.5 containing L-alanine, ketoglutaric acid, a source of inorganic phosphorous, the enzyme pyruvate oxidase, a system for revealing the hydrogen peroxide and optionally one or more co-factors which interact in the enzymatic reaction catalysed by the enzyme pyruvate oxidase. It is moreover described a method for the determination of the enzyme alanine aminotransferase making use of said reagent composition and of the surface antigen of the hepatitis B virus (HBsAg), by enzyme-immune assay, in the same serum specimen and in the same well of a microtitration plate. The method is particularly useful for checking the suitability of the blood intended for transfusions since it can be easily carried out with equipments and instruments widely used for the immunoenzymatic screening of infectivity. Furthermore it enables to test in the same analytical assay the two labels of hepatocyte infections n…
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