Immobilization of enzymes with a cross-linking agent and a polymer containing l-amino ethylene moieties
US5279948A · kind A · utility
3Cited by
2References
25Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 28, 1992 |
| Grant date | Jan 18, 1994 |
| Priority date | — |
| Expiry date | Feb 28, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N11/091
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Physically strong particles containing an immobilized enzyme are prepared for use in a fixed bed-column. The particles are prepared by adding a homopolymer of 1-amino ethylene or a copolymer of 1-amino ethylene and N-vinyl formamide to an aqueous medium containing an enzyme, adding a cross-linking agent to cross-link the enzyme and polymer and cause flocculation, and dewatering, sub-dividing and drying the resultant flocculant. Preferably, the enzyme is glucose isomerase and the cross-linking agent is glutaraldehyde, polyazetidine or diisocyanate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.