Exopeptidase catalyzed site-specific bonding of supports, labels and bioactive agents to proteins
US5279954A · kind A · utility
11Cited by
2References
40Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 30, 1989 |
| Grant date | Jan 18, 1994 |
| Priority date | — |
| Expiry date | Jun 30, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/816
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides a means for attaching a label, support or bioactive agent to a protein with an exopeptidase at a site that is remote from the active site of the protein. More specifically the invention is directed to a method for the attachment of an amino acid, amine and alcohol nucleophile to the carboxyl terminus of a protein. In one embodiment, a labeled nucleophile is attached to a protein such as an antibody. In other embodiments, the invention is directed to a method for the attachment of a protein to an immobilization support and to a method for the attachment of a bioactive agent to a protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.