Modified avidin-biotin technique
US5281521A · kind A · utility
281Cited by
2References
8Claims
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Key dates
| Filing date | Jul 20, 1992 |
| Grant date | Jan 25, 1994 |
| Priority date | — |
| Expiry date | Jul 20, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/962
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for preparing an antigen specific probe is provided by incubating a primary antigen specific monoclonal antibody with a biotinylated secondary antibody to produce a complex of the primary and secondary antibodies. The staining pattern produced by these probes reflects the specificity of the monoclonal antibody in the complex and the labeling of irrelevant, endogenous immunoglobulins is reduced substantially. This novel, indirect immunohistochemical method can be used to study normal and diseased tissues using a variety of monoclonal antibodies.
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