Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof
US5281526A · kind A · utility
17Cited by
8References
20Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 20, 1992 |
| Grant date | Jan 25, 1994 |
| Priority date | — |
| Expiry date | Oct 20, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for the preparation of a purified amylase recovered from a fermentation broth, by the addition of a precipitation agent which is a halide salt, most preferably sodium or potassium chloride. According to a preferred embodiment, a further precipitation agent is added which is an organic compound, most preferably an alkyl ester of 4-hydroxybenzoic acid. The present invention also relates to the purified amylases so obtained and to the uses of these purified amylases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.