Bioluminescence method for the determination of pesticides
US5283180A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 9, 1992 |
| Grant date | Feb 1, 1994 |
| Priority date | — |
| Expiry date | Jan 9, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for the rapid and sensitive determination of organophosphate and carbamate pesticides is disclosed. The method employs an insect brain preparation having a mixture of receptors or enzymes with sites that interact with the organophosphate and carbamate pesticides. The pesticides alter or reduce brain activities of the insect brain preparation which are inversely correlated with pesticide concentration in a test sample. The activity is measured by employing substrates which upon exposure to the insect brain preparation are structurally altered and a light emission reaction is observed. The substrates used are 6-substituted D-Luciferin esters wherein the D-Luciferin ester in the presence of the insect brain preparation is inhibited during hydrolysis while in the presence of the pesticide to be determined. Further the D-Luciferin ester in the presence of the insect brain preparation reacts to cleave the substituted ester group at the 6-position to produce D-Luciferin for further reaction with ATP and luciferase to produce bioluminescence. The bioluminescence can then be measured and compared with a control in order to determine the concentration of a pesticide in the test samp…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.