Method for delivering nucleic acids into cells
US5283185A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Aug 28, 1991 |
| Grant date | Feb 1, 1994 |
| Priority date | — |
| Expiry date | Aug 28, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/88
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for facilitating the transfer of nucleic acids into cells comprising preparing a mixed lipid dispersion of a cationic lipid with a co-lipid in a suitable carrier solvent. The lipid has a structure which includes a lipophilic group derived from cholesterol, a linker bond, a spacer arm including from about 1 to about 20 carbon atoms in a branched or unbranched linear alkyl chain, and a cationic amino group selected from the group consisting of primary, secondary, tertiary and quaternary amino groups. The method further comprises adding the nucleic acids to the dispersion to form a complex. The cells are then treated with the complex. There is also disclosed a novel cationic amphiphile useful for this purpose.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.