Preparative electrophoresis device and method
US5284559A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 16, 1992 |
| Grant date | Feb 8, 1994 |
| Priority date | — |
| Expiry date | Jun 16, 2012 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01D57/02
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A preparative electrophoresis device having a pair of spaced apart plates, a pre-cast electrophoretic gel between the plates, and a semipermeable membrane sealably connected to ends of the plates to define with the plates a collection channel for receiving molecules that have traveled through the gel. Also disclosed are close spacing (less than 2.0 mm) of the plates, an inlet tube and an outlet tube connected to the two plates and communicating with opposite ends of the collection channel, an elongated divider between the plates to divide the gel into a purification region and an indicating region that is not as wide as the purification region and receives marker molecules of known molecular weight to indicate the location in the indicating region of molecules of interest, and pumping electrophoretic buffer into the collection channel at the same rate that it is pumped out.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.