Peptidyl prolyl-cis.trans-isomerase
US5284762A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 19, 1990 |
| Grant date | Feb 8, 1994 |
| Priority date | — |
| Expiry date | Jul 19, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/90
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A yeast PPIase characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro stands for L-proline), (2) exhibiting a single molecular weight of about 17,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 6.2 in the isoelectric focusing, and (4) inhibited by CsA; an E. coli PPIase-.beta. characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro for L-proline), (2) exhibiting a single molecular weight of about 20,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 5.0 in the isoelectric focusing, and (4) no being inhibited by CsA; an E. coli PPIase-.alpha. characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro for L-proline), (2) exhibiting a single molecular weight of about 22,000 daltons …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.