Method for sample analysis using capillary electrophoresis
US5286356A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 21, 1993 |
| Grant date | Feb 15, 1994 |
| Priority date | — |
| Expiry date | Jan 21, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44773
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for performing capillary electrophoresis with improved regularity of migration time of the analytes is disclosed. A capillary electrophoresis system includes a fused silica capillary, a high voltage power supply, electrolyte reservoirs at both ends of the capillary, means for injecting a sample, and a detector. After separation, analytes within the sample are identified by comparing their migration time with the migration time of internal or external standards. Since migration times are dependent on the concentration of the injected sample, identification of unknown analytes can often be difficult or subject to error without the use of such standards. These problems associated with concentration dependent migration time are substantially eliminated by using a combination of high voltage power supply running modes (i.e. constant current, constant voltage or constant power) during the course of the analysis. The control of the high voltage power supply can either be set before a run begins, or controlled directly using feedback from the separation in real time.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.