Patent · US Expired

Method for mammalian cell culture

US5286646A · kind A · utility

23Cited by

9References

12Claims

0Family size

Assignee

Boehringer Mannheim GmbH · DE

Inventors

Michael J. Kearns · Roodepoort, ZA
Michael J. Comer · Bernried, DE
Ulrich Steegmans · Tutzing, DE
Herbert Jungfer · Starnberg, DE

Key dates

Filing date	Sep 20, 1991
Grant date	Feb 15, 1994
Priority date	—
Expiry date	Sep 20, 2011

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12M27/02
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention presents a method for the culturing of mammalian cells. This method involves the use of a bioreactor, which contains a sample of mammalian cells in a culture medium containing large molecules. Positioned inside the bioreactor is a semipermeable membrane which defines a space separated from the bioreactor by the semipermeable membrane. A nutrient medium flows through this separated space and, via virtue of the semipermeable nature of the separating membrane, nutrient pass therethrough into the culture medium, while cellular waste products pass into the separated space. The semipermeable membrane is selected so that the cells and large molecules, such as proteinaceous materials, cannot pass through the membrane, but remain in the bioreactor.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.