Purification of factor IX
US5286849A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jul 14, 1992 |
| Grant date | Feb 15, 1994 |
| Priority date | — |
| Expiry date | Jul 14, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y304/21022
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a process for purifying Factor IX from an impure protein fraction containing Factor IX. The purification process comprises the steps of adding a solvent and a detergent to an impure protein fraction and incubating the solvent/detergent protein solution to inactivate any viral contaminants. Factor IX is purified from the solvent/detergent protein solution by chromatography on a sulfated polysaccharide resin without first removing the solvent and detergent prior to the purification on the sulfated polysaccharide resin. The Factor IX, purified by the process has a specific activity of at least 85 units/mg.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.