Method for detecting, identifying, and quantitating organisms and viruses
US5288611A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Mar 19, 1992 |
| Grant date | Feb 22, 1994 |
| Priority date | — |
| Expiry date | Mar 19, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6888
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method and probes for specifically and sensitively detecting, identifying, and quantitating any organism category or group of organisms containing RNA in a sample is disclosed. The nucleic acids of the organisms present in the sample are brought together with a marked probe comprising nucleic acid molecules which are complementary only to nucleic acid containing sequences complementary to RNA sequences known to be conserved in an organism, category or group of organisms. The probe in sample nucleic acid mixture is incubated under nucleic acid hybridization conditions and then assayed to determine the degree of hybridization that has occurred. Hybridization indicates the presence and identity of the organism, category or group of organisms in the sample. The quantity of RNA present in the sample can be determined and compared to that normally present in the known organisms to determine the number of organisms present. Batteries of sequentially more specific probes can also be utilized.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.