Patent · US Expired

Method of irradiating biological specimens

US5288647A · kind A · utility

40Cited by
5References
6Claims
0Family size

Assignee

Inventors

Key dates

Filing dateApr 17, 1991
Grant dateFeb 22, 1994
Priority date
Expiry dateApr 17, 2011

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T436/25
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A method of irradiating a biological specimen with ultraviolet, in particular a polynucleotide specimen selected from DNA or RNA, or optionally a protein. In the case where the specimen is DNA or RNA, or potentially proteins, the specimen is irradiated to cross-link the specimen to a substrate. In the case where the specimen is DNA, the specimen can also be irradiated to form thymine dimers. The method uses an apparatus which permits relatively precise control of the total ultraviolet dose received by the specimen, despite any changes of ultraviolet flux from the lamps which may occur from during any one experiment, or between a number of experiments. Thus, the method allows relatively highly reproducible results to be obtained.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.