Method for producing and cloning SacII restriction endonuclease and methylase
US5288696A · kind A · utility
2Cited by
21References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 7, 1990 |
| Grant date | Feb 22, 1994 |
| Priority date | — |
| Expiry date | Sep 7, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a method for cloning and producing the SacII restriction endonuclease by 1) introducing the restriction endonuclease gene from Streptomyces achromogenes into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the SacII restriction endonuclease activity, and 3) purifying the SacII restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the SacII restriction endonuclease activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.