Alkaloids of picralima nitida used for treatment of protozoal diseases
US5290553A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 22, 1991 |
| Grant date | Mar 1, 1994 |
| Priority date | — |
| Expiry date | Jul 22, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of preparing substantially purified alkaloids from seeds, stems, uit-rind and bark of a plant selected from Picralima nitida, Gongronema latifolia, Dorstenia multiradiata, Cola attiensis, Rothmania withfieldii and Desmodium gangeticum, for use in the treatment of protozoal diseases, comprising: PA0 pulverizing said plant; PA0 a first solvent, drying the extracted material and re-extracting the dried material with a different solvent; PA0 extracting a fresh sample of said plant with boiling water; PA0 filtering and concentrating the boiling water solvent extracts under reduced pressure; PA0 concentrating the dried extract to a gum and re-extracting said gum with an aqueous acidic HCl solution; PA0 filtering the acidic extract and making it alkaline to a pH of about 9 with a concentration NaOH solution; PA0 extracting the alkaline solution with dichloromethane; PA0 concentrating organic layers of the extracted alkaline solution to dryness under reduced pressure to obtain an alkaloid fraction; and PA0 separating the alkaloid fraction by liquid chromotography-mass spectrometry to obtain substantially purified alkaloids for use in treatment of protozoal diseases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.