Method of assaying immunization stimulating activity
US5290681A · kind A · utility
10Cited by
1References
5Claims
0Family size
Assignee
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Key dates
| Filing date | Feb 27, 1992 |
| Grant date | Mar 1, 1994 |
| Priority date | — |
| Expiry date | Feb 27, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/04
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of assaying immunization stimulating activity comprising (a) immunizing a lymphocyte in vitro by culturing the lymphocyte in contact with an immobilized antigen in a medium containing a test substance and (b) detecting an immunoglobulin, which is produced and secreted into the medium by the immunized lymphocyte and bound to the immobilized antigen, by counting the immunostained spots deposited in situ on the surface of the immobilized antigen.
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