Osteoinductive protein mixtures and purification processes
US5290763A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 22, 1991 |
| Grant date | Mar 1, 1994 |
| Priority date | — |
| Expiry date | Apr 22, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/84
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for purifying bone-derived osteoinductive factors including an ultrafiltration process, an anion exchange process, a cation exchange process, and a reverse phase HPLC process. The ultrafiltration process preferably includes a first ultrafiltration step using a membrane having a nominal molecular weight cutoff of approximately 100 kilodaltons (kD) and a second ultrafiltration step employing a membrane having a nominal molecular weight cutoff of approximately 10 kD. For the anion exchange process, a strongly cationic resin is used, preferably having quaternary amine functional groups. Typically, the eluant for the anion exchange process has a conductivity from about 10,260 micromhos (.mu.mhos) (1.026.times.10.sup.-2 siemens (S)) to about 11,200 .mu.mhos (1.120.times.10.sup.-2 S). For the cation exchange process, a strongly anionic resin is used, preferably having sulfonic acid functional groups. The eluant for the cation exchange process typically has a conductivity from about 39,100 .mu.mhos (3.91.times.10.sup.-2 S) to about 82,700 .mu.mhos (8.27.times.10.sup.-2 S) or more. The HPLC process typically utilizes a column containing hydrocarbon-modified silica packing material.…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.