Rapid process for detection coliform bacteria
US5292644A · kind A · utility
40Cited by
11References
12Claims
0Family size
Inventor
Key dates
| Filing date | Feb 8, 1991 |
| Grant date | Mar 8, 1994 |
| Priority date | — |
| Expiry date | Feb 8, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/849
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A rapid process for detecting pathogenic microorganisms in products for human consumption comprises contacting the microorganisms with a methylumbelliferone substrate. The substrate is hydrolyzed into methylumbelliferone by an enzyme given off by the microorganisms. Hydrolysis is accelerated by sodium lauryl sulfate, which renders the microorganisms more permeable to the substrate, the enzyme, or both. The methylumbelliferone is detected by its fluorescence, either in solution or on an agar medium supporting microcolonies formed from individual microorganisms.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.