Method for cloning and producing the NaeI restriction endonuclease and methylase
US5292651A · kind A · utility
1Cited by
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7Claims
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Key dates
| Filing date | May 12, 1992 |
| Grant date | Mar 8, 1994 |
| Priority date | — |
| Expiry date | May 12, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a method for cloning and producing the NaeI restriction endonuclease by 1) introducing the restriction endonuclease gene from Nocardia aerocolonigenes into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the NaeI restriction endonuclease activity, and 3) purifying the NaeI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the NaeI restriction endonuclease activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.