Agents for the detection of substrates with hydrolase activity

US5292669A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Hans-Joachim Guder · Weilheim, DE
• Werner Guthlein · Mannheim, DE
• Wolfgang Weckerle · Grünstadt, DE
• Johann Berger · Alfdorf, DE
• Harvey Buck · Indianapolis, US
• Rupert Herrmann · Weilheim, DE

Key dates

• Filing date: Aug 10, 1992
• Grant date: Mar 8, 1994
• Expiry date: Aug 10, 2012

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/924
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

A method of detecting hydrolase activity using as substrates the compounds of the formula ##STR1## wherein each of R.sup.1, R.sup.2 and R.sup.3 is C.sub.1 -C.sub.4 alkyl, or phenyl optionally substituted in the meta- or para- position by C.sub.1 -C.sub.6 alkyl, C.sub.1 -C.sub.6 alkoxy or mono- or di-(C.sub.1 -C.sub.6)-alkylamino, or optionally substituted by an O-X group in which X is a glycosyl, phosphate or acyl moiety of a natural substrate of the corresponding glycosidase, phosphatase or esterase.