Method for producing the Hpa I restriction endonuclease and methylase
US5298404A · kind A · utility
1Cited by
11References
7Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 13, 1989 |
| Grant date | Mar 29, 1994 |
| Priority date | — |
| Expiry date | Oct 13, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a method for cloning and producing the Hpa I restriction endonuclease by 1) introducing the restriction endonuclease gene from Haemophilus parainfluenzae into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the Hpa I restriction endonuclease, and 3) purifying the Hpa I restriction endonuclease from the fermented host which contains the vector encoding and expressing the Hpa I restriction endonuclease activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.