Method of differentiating erythroblasts from other cells by flow cytometry
US5298426A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 7, 1991 |
| Grant date | Mar 29, 1994 |
| Priority date | — |
| Expiry date | Nov 7, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/101666
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method which comprises using a specified dye taken up by erythrocytic nucleated cells so that their nuclei are stained and can be readily differentiated from other cells by measurement with a flow cytometer is disclosed. The method relies basically upon flow cytometry by two-step staining that uses a first fluid that is an acidic hypotensive fluorescent dye solution and a second fluid that is a solution that changes the osmorality and pH of the first fluid. The first fluid further contains a fluorescent dye that diffuses into erythroblasts to specifically stain their nuclei, and a group of erythroblasts is separated from other cell groups on the two-dimensional plot constructed with the flow cytometer, whereby the results of erythroblast differentiation are computed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.