Protein assay method
US5300440A · kind A · utility
6Cited by
2References
10Claims
0Family size
Inventor
Key dates
| Filing date | Jun 24, 1992 |
| Grant date | Apr 5, 1994 |
| Priority date | — |
| Expiry date | Jun 24, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6833
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for protein assay is a modified Lowry method in which a Step 1 reaction between a copper-containing alkaline solution and a protein solution takes place at high alkali concentration. The Step 2 reaction the products of Step 1 and Folin reagent commences at a pH of 11 and 12 in order to produce rapidly the colored species on the basis of which a maximum optical density is reached in a relatively short time and remains constant for a sufficient period to enable the required number of optical density determinations to be made.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.