Transient-state luminescence assay apparatus
US5302349A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 6, 1990 |
| Grant date | Apr 12, 1994 |
| Priority date | — |
| Expiry date | Mar 6, 2010 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/6428
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Light, pulsed or continuous at a particular wavelength (e.g. 780 nm), fluoresces a specimen. The specimen may be combinations of an antigen (e.g. rubella) labelled with a fluorescent dye, unlabeled antigen or hapten and an antibody reactive with the antigen or hapten. The light polarized in a first direction (e.g. z-axis) parallel to the electric field of the incident light and in a second direction (e.g. x-axis) perpendicular to the first direction is measured. A second specimen is then provided with the antigen and the antibody but without the dye. The same light as discussed above excites the second specimen and polarizes the light. The light polarized in the first (z-axis) and second (x-axis) directions in the second specimen is measured. These measurements are processed in a microprocessor with the measurements in the z and x directions in the first specimen to identify the antigen or, when the antigen is known, to identify the concentration of the antigen in the first specimen. When the light is pulsed, the measurements are made in a time window beginning after the initiation, and terminating before the end, of the fluorescence of the combination of the dye, the antibody and …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.