Peptide and DNA sequences
US5302697A · kind A · utility
50Cited by
1References
8Claims
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Assignee
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Key dates
| Filing date | May 21, 1993 |
| Grant date | Apr 12, 1994 |
| Priority date | — |
| Expiry date | May 21, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/90
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Secretory leader sequences, for use in secreting heterologous polypeptides in yeast, are formed by fusing part of the human serum albumin pre-sequence or part of the Kluyveromyces lactis killer toxin pre-sequence to the Saccharomyces cerevisiae mating factor alpha-1 KEX2 cleavage recognition site. The resulting fusion leader sequences are: PA1 (a) H.sub.2 N-Met-Lys-Trp-Val-Ser-Phe-Ile-Ser-Leu-Leu-Phe-Leu-Phe-Ser-Ser-Ala-Tyr-Ser- Arg-Ser-Leu-Asp-Lys-Arg-COOH or PA1 (b) H.sub.2 N-Met-Asn-Ile-Phe-Tyr-Ile-Phe-Leu-Phe-Leu-Leu-Ser-Phe-Val-Gln-Gly-Ser-Leu- Asp-Lys-Arg-COOH Conservative variations are also encompassed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.