Patent · US Expired

Method for concentrating and purifying hirudin from leeches

US5304310A · kind A · utility

7Cited by

10References

2Claims

0Family size

Assignee

BASF SE

Inventors

Kurt Lang · Penzberg, DE
Andreas Kreimeyer · Münstermaifeld, DE

Key dates

Filing date	Jul 19, 1992
Grant date	Apr 19, 1994
Priority date	—
Expiry date	Jul 19, 2012

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S530/855
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method for the concentration and purification of biomolecules is described and comprises introducing the biomolecule into an aqueous/organic solvent system and bringing about a phase separation by addition of salts, where the pH at which the concentration takes place, the solvents suitable for the aqueous/organic solvent system, and the nature of the salt are determined in a series of tests one embodiment concerns concentration of hirudin by using propanol, butanol or acetone a the solvent and sodium chloride as the salt.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.