Installation for obtaining plasmids and cosmids
US5304488A · kind A · utility
5Cited by
7References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 13, 1992 |
| Grant date | Apr 19, 1994 |
| Priority date | — |
| Expiry date | Nov 13, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/101
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process of separating plasmids and cosmids from bacterial cells in which they are contained, comprising adding cultured bacterial cells separated from a culture medium to an inclusion gel-forming substance; lysing the bacterial cells; solubilizing the thus lysed bacterial cells; solidifying the inclusion gel-forming substance to form a gel either before or after the step of solubilizing; and applying an electric field to the thus formed gel sufficient to cause electrophoretic migration of the plasmids and cosmids and isolation of the plasmids and cosmids. Also disclosed is a device for carrying out the process.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.