Process for production of restrictocin
US5306626A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Mar 16, 1993 |
| Grant date | Apr 26, 1994 |
| Priority date | — |
| Expiry date | Mar 16, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/849
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for recombinant production in procayotic microorganisms such as E. coli of ribotoxins such as restrictocin, alpha-sarcin and mitogillin are described. Known methods were relatively low yielding and not cost effective for commercial use such as in the pharmaceutical industry where relatively large quantities of toxin with consistent batch to batch quality may be required for immunotoxin production. Use of recombinant methods of production open up the possibility of making ribotoxin analogues. Toxicity of ribotoxins was recognised as a concern in development of a high yielding cost effective production method. Methods for high yielding intracellular accumulation or secretion of ribotoxins are described. Use of protease deficient strains and other methods of minimising breakdown of ribotoxin by protease are preferred. Vectors and host strains for use in the methods are described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.