Method for sequencing double-stranded DNA
US5308751A · kind A · utility
232Cited by
5References
18Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 23, 1992 |
| Grant date | May 3, 1994 |
| Priority date | — |
| Expiry date | Mar 23, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for simultaneously sequencing both strands of a target DNA is provided. The method involves "shifting" one or both strands of the target DNA by addition or deletion of one or more nucleotides to one or both strands of the duplex to produce shifted DNA. Both strands of the shifted DNA duplex and the target DNA are sequenced and the sequences of the shifted and unshifted target DNA are compared. Since portions of the sequenced strands of the target and shifted DNA are identical, all or a portion of sequence of the target DNA can be determined.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.