Methods for purifying and detecting IGM antibodies

US5308753A · kind A · utility

Assignee

• The Government of the United States of America as represented by the Secretary of the Department of Health and Human Services STA MD COD 06 · US

Inventors

• David W. Dorward · Hamilton, US
• Edward D. Huguenel · Guilford, US
• Gary Davis · Milford, US
• Claude F. Garon · Hamilton, US

Key dates

• Filing date: Feb 20, 1992
• Grant date: May 3, 1994
• Expiry date: Feb 20, 2012

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/863
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods for purifying and detecting IgM antibodies employ binding substances which are Borellia burgdorferi cells, or cellular or extracellular components obtained or derived therefrom and which bind to this class of antibodies. The binding substances may be attached to a solid substrate and then the substrate contacted with a solution containing IgM antibodies under conditions such that the antibodies bind to the binding substance on the substrate. The substrate is then contacted with a solution that releases the IgM antibodies from the substrate and the antibodies are recovered or detected. Applications of these methods include, for example, assays for diagnosing diseases which elicit primary and/or secondary IgM antibody-mediated immunity.