Dyes designed for high sensitivity detection of double-stranded DNA
US5312921A · kind A · utility
48Cited by
2References
4Claims
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Key dates
| Filing date | May 14, 1993 |
| Grant date | May 17, 1994 |
| Priority date | — |
| Expiry date | May 14, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44726
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.