Electrophoresis of nucleic acid fragments
US5314595A · kind A · utility
13Cited by
7References
3Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Apr 3, 1992 |
| Grant date | May 24, 1994 |
| Priority date | — |
| Expiry date | Apr 3, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44747
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A method for electrophoresis of nucleic acid fragments present in the solution which contains an amount, e.g., 0.2% or more, of a reagent, e.g., glycerol, dithiothreitol (DTT) and trehalose or other sugars, which interact to form a complex with borate or boric acid. The method includes applying the solution to an electrophoretic gel and electrophoresing those fragments into the gel in the presence of a buffer lacking boric acid, or a derivative thereof, which forms a chelate complex with the reagent and thereby causes distortion of electrophoresis of the fragments in a gel including such a buffer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.