Patent · US Expired

Process and test carrier for the determination of an enzyme from an isoenzyme mixture

US5314803A · kind A · utility

5Cited by

8References

20Claims

0Family size

Assignee

Boehringer Mannheim GmbH · DE

Inventors

Hans-Erich Wilk · Mannheim, DE
Anselm Rothe · Birkenau, DE
Erich Schneider · Tauberbischofsheim, DE

Key dates

Filing date	Dec 10, 1992
Grant date	May 24, 1994
Priority date	—
Expiry date	Dec 10, 2012

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S435/97
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The present invention provides a process for the determination of an enzyme from an isoenzyme mixture in a liquid sample by inhibition of the disturbing isoenzymes and determination of the non-inhibited enzyme, wherein the isoenzyme mixture is contacted with one or more substances which are able to inhibit the disturbing isoenzymes, the sample containing the inhibiting substance(s) is transferred to a small-pored reaction medium and the disturbing enzyme is there inhibited and the determination of the non-inhibited enzyme is carried out in the resulting liquid. The present invention also provides a test carrier for carrying out this process.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.