Apparatus and method for fluorescent lifetime measurement
US5315122A · kind A · utility
61Cited by
5References
15Claims
0Family size
Assignee
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Key dates
| Filing date | Aug 25, 1992 |
| Grant date | May 24, 1994 |
| Priority date | — |
| Expiry date | Aug 25, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/1454
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A flow cytometer measures fluorescence lifetimes by the phase shift of a reference signal and an emission from a fluorescent particle or cell in a flow chamber in order to identify the particle or cell. An acoustic optic modulator modulates laser light with a sinusoidal wave of a predetermined frequency to excite particles or cells. Detectors respond to emissions of individual particles or cells in the form of an output signal pulse at the predetermined frequency. Signals representative of fluorescence emitted and reference beam signals is measured. The particle is identified by the phase shift.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.