Enzymatic fluorometric assay for adenylate cyclase
US5316907A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 22, 1993 |
| Grant date | May 31, 1994 |
| Priority date | — |
| Expiry date | Jan 22, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/811
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of measuring adenylate cyclase (AC) in a sample of physiological material which does not employ radioactive reagents is provided, comprising: PA1 (a) providing a physiological sample of physiological material comprising cAMP produced by endogenous AC, and other endogenous adenine nucleotides selected from the group consisting of ATP, AMP, ADP and mixtures thereof; PA1 (b) combining the sample with effective amounts of apyrase, 5'-nucleotidase and adenosine deaminase, so as to enzymatically eliminate the other endogenous adenine nucleotides in the sample; PA1 (c) enzymatically converting the cAMP into AMP; and PA1 (d) measuring the amount of AMP, the amount providing a measure of the amount of cAMP and AC in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.