Size markers for electrophoretic analysis of DNA
US5316908A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 13, 1990 |
| Grant date | May 31, 1994 |
| Priority date | — |
| Expiry date | Jul 13, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses a DNA marker ladder useful in Southern blot hybridizations. The ladder is made up of pooled DNA restriction endonuclease digests, where each restriction digest contains at least one fragment complementary to a probe and at least one fragment not complementary to the probe. The regions of complementarity between the probe and the complementary fragments are double-stranded segments of the fragments. The ladder is characterized by an approximately even spacing of bands, resulting from choosing fragments having an logarithmic size distribution. Kits incorporating this ladder and a probe or means for making a probe or a probe and a means for labeling a probe are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.