Method for increasing fluorescence
US5316909A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Mar 13, 1992 |
| Grant date | May 31, 1994 |
| Priority date | — |
| Expiry date | Mar 13, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/827
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to a method based on fluorescence, especially time-resolved fluorescence for quantitative assay of a bioaffinity reaction involving bioaffinity components. The method comprises the labelling of one or several of the bioaffinity components participating in the reaction with a lanthanide chelate, forming of a lanthanide chelate for a fluorescence measurement after the reaction, and measuring the fluorescence of the chelate. The lanthanide (Eu, Tb, Sm or Dy) is brought to a strongly fluorescent form before the fluorescence measurement by incorporating the lanthanide in an aggregated particle that comprises the lanthanide chelate and a chelate of a fluorescence-increasing ion (Y, Gd, Tb, Lu or La) to bring about a cofluorescence effect. An aliphatic or aromatic beta-diketone is used as the chelating compound in the aggregate.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.