Patent · US Expired

Defined media for serum-free tissue culture

US5316938A · kind A · utility

74Cited by
3References
1Claims
0Family size

Assignee

Inventors

Key dates

Filing dateDec 18, 1992
Grant dateMay 31, 1994
Priority date
Expiry dateDec 18, 2012

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2501/395
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A biochemically defined culture medium for culturing engineered Chinese hamster ovary (CHO) cell lines, which is essentially free from protein, lipid and carbohydrate isolated from an animal source, comprising water, an osmolality regulator, a buffer, an energy source, amino acids including L-glutamine, an inorganic or recombinant iron source, and a synthetic or recombinant growth factor, and optionally non-ferrous metal ions vitamins and cofactors; also cells adapted to grow in such a culture medium.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.