Staining methods for histology and cytology specimens
US5318795A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 5, 1993 |
| Grant date | Jun 7, 1994 |
| Priority date | — |
| Expiry date | Apr 5, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2001/317
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In the field of staining methods for histology and cytology specimens, the improvements of the invention are three-fold. First included is a drying-clearing step whereby a specimen is cleared by drying. Thus, the use of hazardous chemical-clearing agents to remove washing-dehydrating solution prior to cover-slipping is eliminated. Second, in a staining method using spray staining equipment, the use of a low molarity, combination, bluing-washing solution which serves the dual purpose of a washing solution and a bluing reagent, eliminates the use of water in a staining procedure. Third, and also in a staining method using spray staining equipment, the use of a series of sprays of fresh, essentially anhydrous, washing-dehydrating solution to dehydrate a specimen replaces immersion in a series of washing-dehydrating solutions and thereby decreases the amount of chemical consumed and also avoids contamination of the reagent with water and specimen debris.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.