Assays for inhibitors of leukocyte adhesion

US5318890A · kind A · utility

Assignee

• The Regents of the University of California · US

Inventors

• Steven D. Rosen · San Francisco, US
• Mark S. Singer · Berkeley, US
• Yasuyuki Imai · Tokyo, JP
• Ted Yednock · Grass Valley, US

Key dates

• Filing date: May 6, 1991
• Grant date: Jun 7, 1994
• Expiry date: May 6, 2011

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/33
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention provides novel assays for determining the ability of a test compound to inhibit intercellular adhesion mediated by a selectin receptor, such as the LHR. The assays involve contacting the test compound with the receptor and an isolated receptor-binding agent. The receptor-binding agent is a sulfated polysaccharide, a sulfated glycolipid, or a compound comprising the extracellular region of an endothelial cell surface glycoprotein. Also provided are compositions comprising a compound comprising the extracellular region of an endothelial cell surface protein, which is specifically recognized by lymphocyte homing receptors.