Method for measurement of intracellular ion concentration using fluorescence probe dyes
US5319209A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 19, 1992 |
| Grant date | Jun 7, 1994 |
| Priority date | — |
| Expiry date | Nov 19, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6419
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A fluorescence probe dye is introduced into a cell, and excitation beams at three defferent wavelengths are irradiated to the cell to measure intensities of the fluorescence generated by the excitation beams, corresponding to the three wavelengths. Then, an equilibrium reaction equation for concentrations of the fluorescence probe dye, protein, free ions and their complexes in the cell, and a relationship equation between the fluorescence probe dye, protein, free ions and their complexes are used as simultaneous equations to give concentrations of the respective components. This process can correct interactions among various components of the cell due to bonding among them and a correct ion concentration can be determined.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.