Electrophoresis
US5320727A · kind A · utility
7Cited by
0References
11Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jul 27, 1993 |
| Grant date | Jun 14, 1994 |
| Priority date | — |
| Expiry date | Jul 27, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/28
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of separating or distinguishing proteins by isoelectric focusing (IEF), comprises fluorescently labelling proteins by reacting monobromobimane or monochlorobimane with free sylphydryl groups of proteins, and subjecting the labelled proteins to isoelectric focusing. The IEF may be followed by a second electrophoretic separation step, resulting in 2-D electrophoresis of flourescently pre-labelled proteins in a way that has not hitherto been possible.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.