Cephalosporin C acylase
US5320948A · kind A · utility
12Cited by
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7Claims
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Key dates
| Filing date | Aug 26, 1992 |
| Grant date | Jun 14, 1994 |
| Priority date | — |
| Expiry date | Aug 26, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P35/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A cephalosporin C acylase from Pseudomonas diminuta N-176 is characterized by its ability to catalyze the conversion of cephalosporin C, glutaryl 7-ACA, adipyl 7-ACA, succinyl 7-ACA, N-acetylcephalosporin, N-benzoylcephalosporin C and cephalothin into 7-aminocephalosporanic acid. The enzyme contains an .alpha.-subunit having a molecular weight of 26 kDA and a .beta.-subunit having a molecular weight of 58 kDa. A method for the recombinant production of the present cephalosporin C acylase in Escherichia coli is provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.