Method for cloning restriction modification system
US5320957A · kind A · utility
15Cited by
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16Claims
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Assignee
Inventors
Key dates
| Filing date | Aug 11, 1992 |
| Grant date | Jun 14, 1994 |
| Priority date | — |
| Expiry date | Aug 11, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A two-step method for cloning restriction modification systems is provided which comprises introducing the modification or methylase gene into a host cell and expressing the gene therein to protect the host cell followed by introducing the restriction or endonuclease gene into the host whereby the restriction gene is expressed at lower levels relative to the modification gene. Related methods for producing restriction enzymes is also disclosed as well as clones containing the BamI and DdeI restriction genes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.